Phitotoxicity of crhomium over Phaseolus vulgaris L.

Authors

  • Ernesto Benito Giardina Cátedra de Edafologia, Fac. de Agronomía, UBA.
  • O.S. Heredia Cátedra de Edafologia, Fac. de Agronomía, UBA.
  • M.A. Castro Cátedra de Edafologia, Fac. de Agronomía, UBA.
  • Diana N. Effron Cátedra de Química General, Fac. de Agronomía, UBA giardina@agro.uba.ar

Keywords:

Chromiun (VI), Phaseolus vulgaris, ecotoxicological bioassays

Abstract

Heavy metals contamination and the problems that these generate on the biota have been documented. Chromium is a heavy metal that is not free in nature. It is important determine the level of heavy metals environmental risk on diverse terrestrial ecosystem using ecotoxicological bioassays. The aims of this work is to evaluate the effect of different chromium concentrations on the growth of Phaseolus vulgaris L. That will not only offers information about anatomical variations on the species in study and will be ussefull in future experiments. They carried out germination trial with increasing doses of Cr: 0 (control), of 0.000001; 0.00001; 0.0001; 0.001; 0.01; 0.1; 0.15; 0.2; 0.25; 0.5; 0.75; 1; 1.5; and 2 M, the Cr is apllicated as potassium chromate, by establishing 7 repetitions of Phaseolus vugaris L. seeds for treatment. Groups of four seeds were used in plastic containers and put over filter paper in each replica.For 0; 0.1; 0.75 and 2 M concentrations we carrie out a test in gavels with soil and compost. Both test were carried out in a randomized design. ANVA analysis was maked to evaluate the effect of treatments.All the seeds germinated from concentrations of chromium over 10-2 M equivalent to 520 mg.L-1, this is a higher value that obtained in the bibliography. There were differences between treatments in the radicle and the stem length. The dose of 10-6 M could be the promoter growth of these two organs. There were no difference between the width and length of the leaves, this shows that the traslocación of the chromium in leaves was low. In gavels test the soil had not effect over Cr concentration and seeds have not germinated.

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References

Aportela Gilling, P.L. y Y. González Pérez. 200.1Evaluación toxicológica del dicromato de potasio en plantas de lechuga, Lactuca sativa L. Anuario Toxicología 2001(1): 98-103.

Aurazo de Zumaela, M. y M.L. Esparza. 1995. Toxicidad aguda del cromo usando Allium cepa L. OPS/CEPIS/95-25(LAB). 23pp.

Giardina, E.B.; O.S. Heredia; J. Chirkes; L. Ciuffoli y M. Auget. 2010. Valoracion de la toxicidad del cromo sobre la germinación y el crecimiento de distintas especies de importancia horticola. Congreso SETAC: 24.

Gunse Forcadell, B. 1987. Efecto del cromo sobre la nutrición y las relaciones hídricas de Phaseolus vulgaris L. Tesis doctoral, Universidad Autónoma de Barcelona, España, 209pp.

Huffman, E.W.D, and W.H. Alloway. 1973. Growth of plants in solution culture containing low levels of chromium. Plant Phisisl.,52 : 72-75.

Iannacone, O.J. y F.A. Alvariño. 2005 Efecto Ecotoxicológico de tres Metales Pesados Sobre el Crecimiento Radicular de Cuatro Plantas Vasculares. Agric. Técnica vol 65(2): 198-203.

Judith, P.M.; C.A. González Ramírez; A.D. Román Gutiérrez y F. Prieto García. 2009. Contaminación y Fototoxicidad en plantas por metales pesados provenientes de suelos y agua. Tropical and Subtropical Agroecosystems 10: 29-44.

Meagher, R.B. 2000. Phitorremediation of toxic elemental and organic pollutants. Curr. Opin Plant Biol., 3: 153-162.

OECD, 1984. Terrestrial plant: Growth test OECD. Guideline for chemicals Nº 208. Organization for Economic ooperation and Development, Paris.

Otiniano García, M.; L. Tuesta Collantes; H. Robles Castillo; M. Luján Velásquez y M. Chávez Castillo. 2007. Biorremediación de cromo VI de aguas residuales de curtiembres por Pseudomonas sp y su efecto sobre el ciclo celular de Allium cepa. Rev. Med. Vallejiana v.4 (1) Lima: 32-42.

Peralta J.R.; J.L. Gardea-Torresdey; K.J. Tiemann; E. Gómez; S. Arteaga; E. Rasscon and J.G. Parsons. 2001. Uptake and effects of five heavy metals on seed germination and plant growd in Alfalfa (Medicado sativa L.).

Rosa, C.E.V.; M. Sierra and C.M. Radetski. 1999. Use of plant test in the evaluation of textile effluent toxicity. Ecotoxicology Environmental Research 2 : 56-61.

Wang, W. and J.M. Williams. 1990. The use of phytotoxicity test (common dockweed, cabbage, and millet) for determining effluent toxicity. Environ. Monit. Asses. 14: 45-58.

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Published

2013-05-14

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