Chlorophyll content estimation in oregano leaves using a portable Chlorophyll meter : relationship with Mesophyll thickness and leaf age

Authors

  • Marcos Sebastian Pereyra Laboratorio de Fisiología Vegetal, Depto. de Producción Vegetal, Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba.
  • Vanina Davidenco Laboratorio de Fisiología Vegetal, Depto. de Producción Vegetal, Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba.
  • Selva Beatriz Núñez Laboratorio de Fisiología Vegetal, Depto. de Producción Vegetal, Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba.
  • Juan Alberto Argüello Laboratorio de Fisiología Vegetal, Depto. de Producción Vegetal, Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba.

Keywords:

origanum vulgare, CCM 200 plus, thickness index, spongy parenchyma

Abstract

Chlorophyll meter CCM 200 plus capacity to estimate total leaf chlorophyll content (Chl) in Origanum vulgare ssp. hirtum was evaluated. This study aimed to elaborate a calibration model adapted to the species and evaluate the effect of mesophyll thickness and leaf age on the readings. This research included a wide range of chlorophyll concentrations, mesophyll thicknesses and leaves from different layers within the plant. Thus good estimates of total chlorophyll content were obtained with this meter, although it was less accurate in mature leaves, which have higher values of chlorophyll content and mesophyll thickness. As mesophyll thickness increases, spongy parenchyma develops more than other anatomical structures, modifying the leaf optical properties and increasing the variability of the readings. The best fit was obtained with a logistic equation [Chl=0.343/(1+1.925(-0.069x))], adapted to a broad data ranges and any leaf typology, according to ontogenetic age and position in the plant.

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Author Biography

  • Marcos Sebastian Pereyra, Laboratorio de Fisiología Vegetal, Depto. de Producción Vegetal, Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba.
      

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Published

2014-11-30

Issue

Vol. 34 No. 1-2 (2014)

Section

Artículos

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